jarid2 (Cell Signaling Technology Inc)
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Jarid2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+jarid2/SUZ12+XP+Rabbit+mAb/pm41785858-812-34-35
Average 96 stars, based on 407 article reviews
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Chromatin Immunoprecipitation:Article Title: Accessory subunits of PRC2 mimic H3K27me3 to restrict the spread of Polycomb domains. Article Snippet: .. The antibodies used for ChIP included: anti-SUZ12 (Cell Signaling 3737, 0.42 μg), anti-H3K27me3 (Cell Signaling 35861SF, 3 μg) Article Title: Accessory subunits of PRC2 mimic H3K27me3 to restrict the spread of Polycomb domains Article Snippet: .. The antibodies used for ChIP included: anti-SUZ12 (Cell Signalling 3737, 0.42 μg), anti-H3K27me3 (Cell Signalling 35861SF, 3 μg) Transferring:Article Title: EZH2 variants derived from cryptic splice sites govern distinct epigenetic patterns during embryonic development Article Snippet: .. After transferring proteins onto polyvinylidene fluoride (PVDF) membranes (Millipore, #IPVH00010), the membranes were blocked with 5% nonfat milk and incubated overnight at 4°C with specific primary antibodies, including anti-FLAG (Sigma #F3165), anti-HA (CST, #3724), anti-SUZ12 (CST, #3737), anti-EED (CST, 51673), Incubation:Article Title: EZH2 variants derived from cryptic splice sites govern distinct epigenetic patterns during embryonic development Article Snippet: .. After transferring proteins onto polyvinylidene fluoride (PVDF) membranes (Millipore, #IPVH00010), the membranes were blocked with 5% nonfat milk and incubated overnight at 4°C with specific primary antibodies, including anti-FLAG (Sigma #F3165), anti-HA (CST, #3724), anti-SUZ12 (CST, #3737), anti-EED (CST, 51673), Article Title: Distinct specificity and functions of PRC2 subcomplexes in human stem cells and cardiac differentiation Article Snippet: For each IP, 1 mL chromatin dilution buffer (25 mM Tris pH 7.5, 5 mM EDTA, 1% Triton X-100, 0.1% SDS, 1X protease inhibitor cocktail (Thermo Fisher Scientific, #A37989)) together with 20 ng spike-in chromatin from Drosophila melanogaster S2 cells (Active Motif, #53083) was added to the cleared lysate from 2.5–5.0 million cells. .. Chromatin samples were incubated with 2–4 μg of anti-CBX7 (Abcam, #ab21873, 4 μL), anti-FLAG (Millipore Sigma, #F3165, 2 μL), anti-H2AK119ub (Cell Signaling Technology, #8240, 5 μL), anti-H3K27me3 (Cell Signaling Technology, #9733, 6 μL), anti-H3K36me3 (Thermo Fisher Scientific, #MA5–24687, 3 μL), anti-H3K4me3 (Thermo Fisher Scientific, #MA5–33382, 4 μL), Western Blot:Article Title: Accessory subunits of PRC2 mimic H3K27me3 to restrict the spread of Polycomb domains. Article Snippet: Densitometry of the bands was measured using BioRad Image Lab. .. The antibodies used for immunoblotting included: anti-GAPDH (Proteintech 10494-1-AP, 1:4000), anti-H3 (Abcam #Ab1791, 1:100000), anti-H3K27me1 (Active Motif 61015, 1:2000), anti-H3K27me2 (Abcam ab24684, 1:1000), anti-H3K27me3 (Active Motif 61017, 1:2500), anti-EZH2 (Cell Signaling 5246S, 1:1000), anti-SUZ12 (Cell Signaling 3737, 1:1000), anti-EED (Cell Signaling 85322, 1:1000), anti-LCOR (Merck #ABE1367, 1:250), |
