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jarid2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc jarid2
    Jarid2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+jarid2/SUZ12+XP+Rabbit+mAb/pm41785858-812-34-35
    Average 96 stars, based on 407 article reviews
    jarid2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Accessory subunits of PRC2 mimic H3K27me3 to restrict the spread of Polycomb domains.
    Article Snippet: .. The antibodies used for ChIP included: anti-SUZ12 (Cell Signaling 3737, 0.42 μg), anti-H3K27me3 (Cell Signaling 35861SF, 3 μg) anti-Jarid2 (Cell Signaling 13594S, 1.115 μg), anti-MTF2 (ProteinTech 16208-1-AP, 1.435 μg), anti-H3K4me3 (Cell Signaling 9751S, 0.605 μg), anti-H2AK119ub (Cell Signaling 8240S, 2.69 μg), and anti-RING1B (Cell Signaling 5694S, 1.095 μg). .. After the antibody incubation, the lysate was centrifuged at max speed for 30 minutes at 4 ◦ C. The pellet was discarded and 40 μL of Dynabeads Protein G (Thermo #10003D), washed 5 times in ChIP buffer, were added to the supernatant of each sample and incubated for 2–4 hours at 4 ◦ C rotating at low e8 Molecular Cell 86, 1–14.e1–e10, March 19, 2026 speed.

    Article Title: Accessory subunits of PRC2 mimic H3K27me3 to restrict the spread of Polycomb domains
    Article Snippet: .. The antibodies used for ChIP included: anti-SUZ12 (Cell Signalling 3737, 0.42 μg), anti-H3K27me3 (Cell Signalling 35861SF, 3 μg) anti- Jarid2 (Cell Signalling 13594S, 1.115 μg), and anti-MTF2 (Proteintech 16208-1-AP, 1.435 μg). ..

    Transferring:

    Article Title: EZH2 variants derived from cryptic splice sites govern distinct epigenetic patterns during embryonic development
    Article Snippet: .. After transferring proteins onto polyvinylidene fluoride (PVDF) membranes (Millipore, #IPVH00010), the membranes were blocked with 5% nonfat milk and incubated overnight at 4°C with specific primary antibodies, including anti-FLAG (Sigma #F3165), anti-HA (CST, #3724), anti-SUZ12 (CST, #3737), anti-EED (CST, 51673), anti-JARID2 (CST, #13594), anti-AEBP2 (CST, #14129), anti-EZH2 (CST, #5246), anti-H3K27me3 (CST, #9733), anti-H3K27me2 (CST, #9728), anti-H3K27me1 (CST, #17186), anti-H3K27ac (CST, #8173) and anti-H3 (CST, #4499), anti-YAP1 (CST, #8418), anti-TEAD1 (CST, #12292), anti-ERK (CST, #9102), anti-p-ERK (CST, #44370), anti-JNK (CST, #9252), anti-p-JNK (CST, #9251), anti-p38 (CST, #8690), anti-p-p38 (CST, #4511), and anti-GAPDH (CST, #2118). .. The membranes were then incubated with a secondary antibody and visualized using ECL Western Blotting Detection Kit (ThermoFisher, #32109).

    Incubation:

    Article Title: EZH2 variants derived from cryptic splice sites govern distinct epigenetic patterns during embryonic development
    Article Snippet: .. After transferring proteins onto polyvinylidene fluoride (PVDF) membranes (Millipore, #IPVH00010), the membranes were blocked with 5% nonfat milk and incubated overnight at 4°C with specific primary antibodies, including anti-FLAG (Sigma #F3165), anti-HA (CST, #3724), anti-SUZ12 (CST, #3737), anti-EED (CST, 51673), anti-JARID2 (CST, #13594), anti-AEBP2 (CST, #14129), anti-EZH2 (CST, #5246), anti-H3K27me3 (CST, #9733), anti-H3K27me2 (CST, #9728), anti-H3K27me1 (CST, #17186), anti-H3K27ac (CST, #8173) and anti-H3 (CST, #4499), anti-YAP1 (CST, #8418), anti-TEAD1 (CST, #12292), anti-ERK (CST, #9102), anti-p-ERK (CST, #44370), anti-JNK (CST, #9252), anti-p-JNK (CST, #9251), anti-p38 (CST, #8690), anti-p-p38 (CST, #4511), and anti-GAPDH (CST, #2118). .. The membranes were then incubated with a secondary antibody and visualized using ECL Western Blotting Detection Kit (ThermoFisher, #32109).

    Article Title: Distinct specificity and functions of PRC2 subcomplexes in human stem cells and cardiac differentiation
    Article Snippet: For each IP, 1 mL chromatin dilution buffer (25 mM Tris pH 7.5, 5 mM EDTA, 1% Triton X-100, 0.1% SDS, 1X protease inhibitor cocktail (Thermo Fisher Scientific, #A37989)) together with 20 ng spike-in chromatin from Drosophila melanogaster S2 cells (Active Motif, #53083) was added to the cleared lysate from 2.5–5.0 million cells. .. Chromatin samples were incubated with 2–4 μg of anti-CBX7 (Abcam, #ab21873, 4 μL), anti-FLAG (Millipore Sigma, #F3165, 2 μL), anti-H2AK119ub (Cell Signaling Technology, #8240, 5 μL), anti-H3K27me3 (Cell Signaling Technology, #9733, 6 μL), anti-H3K36me3 (Thermo Fisher Scientific, #MA5–24687, 3 μL), anti-H3K4me3 (Thermo Fisher Scientific, #MA5–33382, 4 μL), anti-JARID2 (Cell Signaling Technology, #13594, 4 μL), anti-MTF2 (Proteintech, #16208–1-AP, 5 μL), anti-RING1B (Cell Signaling Technology, #5694, 7 μL), or anti-SUZ12 (Cell Signaling Technology, #3737, 4 μL) antibody at 4°C overnight. .. A spike-in antibody against the Drosophila-specific histone variant H2Av (Active Motif, #61686) was added to all chromatin samples.

    Western Blot:

    Article Title: Accessory subunits of PRC2 mimic H3K27me3 to restrict the spread of Polycomb domains.
    Article Snippet: Densitometry of the bands was measured using BioRad Image Lab. .. The antibodies used for immunoblotting included: anti-GAPDH (Proteintech 10494-1-AP, 1:4000), anti-H3 (Abcam #Ab1791, 1:100000), anti-H3K27me1 (Active Motif 61015, 1:2000), anti-H3K27me2 (Abcam ab24684, 1:1000), anti-H3K27me3 (Active Motif 61017, 1:2500), anti-EZH2 (Cell Signaling 5246S, 1:1000), anti-SUZ12 (Cell Signaling 3737, 1:1000), anti-EED (Cell Signaling 85322, 1:1000), anti-LCOR (Merck #ABE1367, 1:250), anti-JARID2 (Cell Signaling 13594S, 1:1000), anti-LAMINB1 (Abcam ab16048, 1:10000), anti-mouse-HRP-conjugated (Jackson ImmunoResearch #715-035-150, 1:5000), and anti-rabbit HRP-conjugated (Santa Cruz Biotechnology #sc-2357, 1:5000). .. Approximately 3–4 × 104 cells were cytospun onto glass slides and allowed to air-dry slightly before fixation in 4% paraformaldehyde solution (PFA) in PBS (Santa Cruz sc-281692) for 15 min at room temperature.



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    Cell Signaling Technology Inc jarid2 asp1114
    (a) Longitudinal analysis of human CD45+ chimerism in peripheral blood (PB) of NSG mice transplanted with cord blood CD34+ cells targeted with guide RNAs for AAVS1 (n=4), <t>JARID2</t> #1 (n=5) or JARID2 #2 (n=5). (b) Longitudinal analysis of modified alleles from CRISPR edits in human CD45+ PB cells in transplanted NSG mice. (c) Lineage composition of human CD45+ PB cells one-year post-transplant (B-cells = CD19+, T-cells = CD3+, myeloid cells = CD33+). (d) Complete blood count (CBC) analysis of white blood cells (WBC), red blood cells (RBC), hemoglobin (Hb), hematocrit (HCT) and platelets (PLT) of recipient mice one-year post-transplant. Dashed lines represent normal range. (e) Representative histology of PB smear, bone marrow (BM) cytospins, and spleen (SPL) harvested one-year after transplantation of CRISPR-edited human CD34+ cells into NSG mice. Scale bars - 10µm (PB) and 25µm (BM and SPL).
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    Image Search Results


    (a) Longitudinal analysis of human CD45+ chimerism in peripheral blood (PB) of NSG mice transplanted with cord blood CD34+ cells targeted with guide RNAs for AAVS1 (n=4), JARID2 #1 (n=5) or JARID2 #2 (n=5). (b) Longitudinal analysis of modified alleles from CRISPR edits in human CD45+ PB cells in transplanted NSG mice. (c) Lineage composition of human CD45+ PB cells one-year post-transplant (B-cells = CD19+, T-cells = CD3+, myeloid cells = CD33+). (d) Complete blood count (CBC) analysis of white blood cells (WBC), red blood cells (RBC), hemoglobin (Hb), hematocrit (HCT) and platelets (PLT) of recipient mice one-year post-transplant. Dashed lines represent normal range. (e) Representative histology of PB smear, bone marrow (BM) cytospins, and spleen (SPL) harvested one-year after transplantation of CRISPR-edited human CD34+ cells into NSG mice. Scale bars - 10µm (PB) and 25µm (BM and SPL).

    Journal: bioRxiv

    Article Title: JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow Transplantation

    doi: 10.1101/2025.09.03.672211

    Figure Lengend Snippet: (a) Longitudinal analysis of human CD45+ chimerism in peripheral blood (PB) of NSG mice transplanted with cord blood CD34+ cells targeted with guide RNAs for AAVS1 (n=4), JARID2 #1 (n=5) or JARID2 #2 (n=5). (b) Longitudinal analysis of modified alleles from CRISPR edits in human CD45+ PB cells in transplanted NSG mice. (c) Lineage composition of human CD45+ PB cells one-year post-transplant (B-cells = CD19+, T-cells = CD3+, myeloid cells = CD33+). (d) Complete blood count (CBC) analysis of white blood cells (WBC), red blood cells (RBC), hemoglobin (Hb), hematocrit (HCT) and platelets (PLT) of recipient mice one-year post-transplant. Dashed lines represent normal range. (e) Representative histology of PB smear, bone marrow (BM) cytospins, and spleen (SPL) harvested one-year after transplantation of CRISPR-edited human CD34+ cells into NSG mice. Scale bars - 10µm (PB) and 25µm (BM and SPL).

    Article Snippet: The following antibodies were used for profiling chromatin-bound factors: anti-CTCF (Cell Signaling Technology, #2899S), EZH2 (Abcam, #ab3748; CST, #5246S), H2AK119ub1 (CST, #8240S), histone H3 (CST, #4499S), H3K27ac (CST, #8173S), H3K27me3 (CST, #9733S), H3K4me3 (CST, #9751S; EpiCypher, #13-0060), and JARID2 (Asp1114) (CST, #13594S).

    Techniques: Modification, CRISPR, Transplantation Assay

    (a) Peripheral blood (PB) engraftment of cord blood cells transduced with indicated constitutive lentiviral shRNAs. (b) Lineage composition of GFP+ PB cells 16-weeks post-transplant. (c) Engraftment of GFP+ human cells in the BM of recipient mice 20-weeks post-transplant. (d) Quantification of GFP+ human HSPC populations in the BM of recipient mice 20-weeks post-transplant (sh Ren n=16, sh JARID2 #1 n=14, sh JARID2 #3 n=17). (e) PB engraftment of cord blood cells transduced with indicated constitutive lentiviral shRNAs in secondary recipients. (f) Lineage composition of GFP+ PB cells 16-weeks post-secondary transplant. (g) Engraftment of GFP+ human cells in the BM of secondary recipient mice 20-weeks post-transplant. (h) Quantification of GFP+ human HSPC populations in the BM of recipient mice 20-weeks post-transplant (sh Ren n=8, sh JARID2 #1 n=6, sh JARID2 #3 n=6).

    Journal: bioRxiv

    Article Title: JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow Transplantation

    doi: 10.1101/2025.09.03.672211

    Figure Lengend Snippet: (a) Peripheral blood (PB) engraftment of cord blood cells transduced with indicated constitutive lentiviral shRNAs. (b) Lineage composition of GFP+ PB cells 16-weeks post-transplant. (c) Engraftment of GFP+ human cells in the BM of recipient mice 20-weeks post-transplant. (d) Quantification of GFP+ human HSPC populations in the BM of recipient mice 20-weeks post-transplant (sh Ren n=16, sh JARID2 #1 n=14, sh JARID2 #3 n=17). (e) PB engraftment of cord blood cells transduced with indicated constitutive lentiviral shRNAs in secondary recipients. (f) Lineage composition of GFP+ PB cells 16-weeks post-secondary transplant. (g) Engraftment of GFP+ human cells in the BM of secondary recipient mice 20-weeks post-transplant. (h) Quantification of GFP+ human HSPC populations in the BM of recipient mice 20-weeks post-transplant (sh Ren n=8, sh JARID2 #1 n=6, sh JARID2 #3 n=6).

    Article Snippet: The following antibodies were used for profiling chromatin-bound factors: anti-CTCF (Cell Signaling Technology, #2899S), EZH2 (Abcam, #ab3748; CST, #5246S), H2AK119ub1 (CST, #8240S), histone H3 (CST, #4499S), H3K27ac (CST, #8173S), H3K27me3 (CST, #9733S), H3K4me3 (CST, #9751S; EpiCypher, #13-0060), and JARID2 (Asp1114) (CST, #13594S).

    Techniques: Transduction

    (a) tSNE plots of lineage distribution of human CD45+ cells in BM of recipient mice 20-weeks post-transplant by flow cytometry. (b) Frequency of maturing myeloid cells in NSGS mice transplanted with cord blood cells transduced with indicated constitutive lentiviral shRNAs 20-weeks post-transplant. (c) JARID2 expression across indicated cell populations showing knockdown efficiency. (d) UMAP of scRNA-seq data for hCD34+ cells isolated 20-weeks post-transplantation. Cell populations are identified by marker genes. (e) Numbers of cells of indicated genotypes per cluster based on Azimuth cell type annotations.

    Journal: bioRxiv

    Article Title: JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow Transplantation

    doi: 10.1101/2025.09.03.672211

    Figure Lengend Snippet: (a) tSNE plots of lineage distribution of human CD45+ cells in BM of recipient mice 20-weeks post-transplant by flow cytometry. (b) Frequency of maturing myeloid cells in NSGS mice transplanted with cord blood cells transduced with indicated constitutive lentiviral shRNAs 20-weeks post-transplant. (c) JARID2 expression across indicated cell populations showing knockdown efficiency. (d) UMAP of scRNA-seq data for hCD34+ cells isolated 20-weeks post-transplantation. Cell populations are identified by marker genes. (e) Numbers of cells of indicated genotypes per cluster based on Azimuth cell type annotations.

    Article Snippet: The following antibodies were used for profiling chromatin-bound factors: anti-CTCF (Cell Signaling Technology, #2899S), EZH2 (Abcam, #ab3748; CST, #5246S), H2AK119ub1 (CST, #8240S), histone H3 (CST, #4499S), H3K27ac (CST, #8173S), H3K27me3 (CST, #9733S), H3K4me3 (CST, #9751S; EpiCypher, #13-0060), and JARID2 (Asp1114) (CST, #13594S).

    Techniques: Flow Cytometry, Transduction, Expressing, Knockdown, Isolation, Transplantation Assay, Marker

    (a) Peripheral blood (PB) engraftment of cord blood cells transduced with indicated inducible lentiviral shRNAs induced for eight-days ex vivo prior to transplant. (b) Lineage composition of hD45+ PB cells 16-weeks post-transplant. (c) Engraftment of hCD45+ cells in the BM of recipient mice 20-weeks post-transplant. (d) Quantification of hCD45+ HSPC populations in the BM of recipient mice 20-weeks post-transplant (sh Ren n=9, sh JARID2 #1 n=6, sh JARID2 #3 n=8, sh EZH2 n=7). (e) PB engraftment of cord blood cells transduced with indicated inducible shRNAs induced in vivo at 14-weeks post-transplant (normalized to sh Ren ). (f) Engraftment of BFP+ hCD45+ cells in the BM of recipient mice (shRNAs for JARID2 and EZH2 are combined for statistical comparison). (g) Quantification of BFP+ hCD45+ HSPC populations in the BM of recipient mice (sh Ren n=3, sh JARID2 #1+3 n=9, sh EZH2 #1+2 n=8).

    Journal: bioRxiv

    Article Title: JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow Transplantation

    doi: 10.1101/2025.09.03.672211

    Figure Lengend Snippet: (a) Peripheral blood (PB) engraftment of cord blood cells transduced with indicated inducible lentiviral shRNAs induced for eight-days ex vivo prior to transplant. (b) Lineage composition of hD45+ PB cells 16-weeks post-transplant. (c) Engraftment of hCD45+ cells in the BM of recipient mice 20-weeks post-transplant. (d) Quantification of hCD45+ HSPC populations in the BM of recipient mice 20-weeks post-transplant (sh Ren n=9, sh JARID2 #1 n=6, sh JARID2 #3 n=8, sh EZH2 n=7). (e) PB engraftment of cord blood cells transduced with indicated inducible shRNAs induced in vivo at 14-weeks post-transplant (normalized to sh Ren ). (f) Engraftment of BFP+ hCD45+ cells in the BM of recipient mice (shRNAs for JARID2 and EZH2 are combined for statistical comparison). (g) Quantification of BFP+ hCD45+ HSPC populations in the BM of recipient mice (sh Ren n=3, sh JARID2 #1+3 n=9, sh EZH2 #1+2 n=8).

    Article Snippet: The following antibodies were used for profiling chromatin-bound factors: anti-CTCF (Cell Signaling Technology, #2899S), EZH2 (Abcam, #ab3748; CST, #5246S), H2AK119ub1 (CST, #8240S), histone H3 (CST, #4499S), H3K27ac (CST, #8173S), H3K27me3 (CST, #9733S), H3K4me3 (CST, #9751S; EpiCypher, #13-0060), and JARID2 (Asp1114) (CST, #13594S).

    Techniques: Transduction, Ex Vivo, In Vivo, Comparison

    (a) UMAP of scRNA-seq data for hCD34+ cells sorted from NSG mice transplanted with cord blood cells transduced with constitutive lentiviral shRNAs 20-weeks post-transplant. Cell populations are identified by marker genes. sh Ren , sh JARID2 #1 and sh JARID2 #3 samples are combined, the HSC population is highlighted. (b) Dot plot highlighting differential expression of a curated HSC signature gene set (Aguadé-Gorgorió et al., 2024, Nature) amongst shRNA genotypes within the HSC population. (c) HSC module scores calculated using the Seurat package based on the curated HSC signature gene list. (d) Serial replating showing CFU efficiency of indicated HSPC populations isolated from BM of recipient mice 20-weeks post-transplant. (e) Volcano plot showing differentially expressed genes between sh JARID2 and sh Ren cells within the HSC population. (f) Representative flow cytometry plots showing the proportion of MHCII-high (red boxes) HSCs from each genotype from the BM of recipient mice 20-weeks post-transplant. (g) Serial replating showing CFU efficiency of indicated HSPC genotypes separated by MHCII levels isolated from BM of recipient mice 20-weeks post-transplant. (h) Cell cycle analysis by flow cytometry showing proportion of quiescent (G 0 ) HSCs of each genotype from the BM of recipient mice 20-weeks post-transplant.

    Journal: bioRxiv

    Article Title: JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow Transplantation

    doi: 10.1101/2025.09.03.672211

    Figure Lengend Snippet: (a) UMAP of scRNA-seq data for hCD34+ cells sorted from NSG mice transplanted with cord blood cells transduced with constitutive lentiviral shRNAs 20-weeks post-transplant. Cell populations are identified by marker genes. sh Ren , sh JARID2 #1 and sh JARID2 #3 samples are combined, the HSC population is highlighted. (b) Dot plot highlighting differential expression of a curated HSC signature gene set (Aguadé-Gorgorió et al., 2024, Nature) amongst shRNA genotypes within the HSC population. (c) HSC module scores calculated using the Seurat package based on the curated HSC signature gene list. (d) Serial replating showing CFU efficiency of indicated HSPC populations isolated from BM of recipient mice 20-weeks post-transplant. (e) Volcano plot showing differentially expressed genes between sh JARID2 and sh Ren cells within the HSC population. (f) Representative flow cytometry plots showing the proportion of MHCII-high (red boxes) HSCs from each genotype from the BM of recipient mice 20-weeks post-transplant. (g) Serial replating showing CFU efficiency of indicated HSPC genotypes separated by MHCII levels isolated from BM of recipient mice 20-weeks post-transplant. (h) Cell cycle analysis by flow cytometry showing proportion of quiescent (G 0 ) HSCs of each genotype from the BM of recipient mice 20-weeks post-transplant.

    Article Snippet: The following antibodies were used for profiling chromatin-bound factors: anti-CTCF (Cell Signaling Technology, #2899S), EZH2 (Abcam, #ab3748; CST, #5246S), H2AK119ub1 (CST, #8240S), histone H3 (CST, #4499S), H3K27ac (CST, #8173S), H3K27me3 (CST, #9733S), H3K4me3 (CST, #9751S; EpiCypher, #13-0060), and JARID2 (Asp1114) (CST, #13594S).

    Techniques: Transduction, Marker, Quantitative Proteomics, shRNA, Isolation, Flow Cytometry, Cell Cycle Assay